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[519] some of his most intelligent and sympathetic listeners in the working class. Now that he needed their assistance he often found his colaborers among farmers, stock-raisers, seafaring men, fishermen, and sailors. Many a New England captain, when he started on a cruise, had on board collecting cans, furnished by Agassiz, to be filled in distant ports or nearer home, as the case might be, and returned to the Museum at Cambridge. One or two letters, written to scientific friends at the time the above-mentioned circular was issued,will give an idea of the way in which Agassiz laid out such investigations.

To James D. Dana.

Cambridge, July 8, 1853.
. . . I have been lately devising some method of learning how far animals are truly autochthones, and how far they have extended their primitive boundaries. I will attempt to test that question with Long Island, the largest of all the islands along our coast. For this purpose I will for the present limit myself to the fresh-water fishes and shells, and for the sake of comparison I will try to collect carefully all the species living in the rivers of Connecticut, New York, and New Jersey, and


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